Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. However, Fluo-3 AM and Fluo-4 AM are only moderately fluorescent in live cells upon esterase hydrolysis, and require harsh cell loading conditions to maximize their cellular calcium responses.
Fluo-8® dyes are developed to improve cell loading and calcium response, while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelength:
4x Brighter than Fluo-3 AM
2x Brighter than Fluo-4 AM
Dye Loading at Room Temperature
Loading Time 30-45 Minutes Only
Enables Calcium Assays with Difficult Receptors and Cell Lines
Excitation at 490 nm
Fluorescence at 520 nm
FITC Filter Set-Compatible
We offer a set of outstanding Fluo-8® reagents with different calcium binding affinities:
Rhod-2 is most commonly used among the red fluorescent calcium indicators. However, Rhod-2 AM is only moderately fluorescent in live cells upon esterase hydrolysis, and has very small cellular calcium responses.
Quest Rhod-4™ has been developed to improve Rhod-2 cell loading and calcium response while maintaining the spectral wavelength of Rhod-2. In CHO and HEK cells Quest Rhod-4™ AM has cellular calcium response that is 10 times more sensitive than Rhod-2 AM.
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